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KMID : 0861020090240010151
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Korea Journal of Herbology
2009 Volume.24 No. 1 p.151 ~ p.157
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Effect of Water Extract from Artemisiae Argi Folium on Mouse Macrophage Stimulated by LPS
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Park Wan-Su
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Abstract
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Objectives: The purpose of this study is to investigate the effect of Water Extract from Artemisiae Argi Folium (WAAF) on mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS).
Methods: Cell viabilities were measured by MTT assay. And the intracellular productions of hydrogen peroxide (H2O2) were measured by dihydrorhodamine 123 assay. TNF- and IL-6 production from Raw 264.7 were measured by ELISA method.
Results: The results of the experiment are as follows. 1. WAAF significantly increased the cell viability compared to the control group (treated with LPS only) at the concentrations of 10, 50, 100, 200, 400 ug/mL. 2. WAAF significantly increased the intracellular production of H2O2 compared to the control group at the concentrations of 50, 100, 200 ug/mL. 3. WAAF significantly decreased the production of TNF- compared to the control group at the concentrations of 100, 200 ug/mL. 4. WAAF significantly decreased the production of IL-6 compared to the control group at the concentrations of 50, 100, 200 ug/mL.
Conclusions: WAAF could be supposed to have the immune-modulating activity related with the macrophage’s immunoactivity.
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KEYWORD
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Macrophage, Artemisiae Argi Folium, Hydrogen Peroxide, TNF-, IL-6
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